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ALPP Antibody (N-term)(Ascites)

Mouse Monoclonal Antibody (Mab)

  • IF - ALPP Antibody (N-term)(Ascites) AM2022a
    Fluorescent confocal image of HeLa cells stained with ALPP (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM2022a ALPP (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ALPP immunoreactivity is localized to the cytoplasm of HeLa cells.
  • WB - ALPP Antibody (N-term)(Ascites) AM2022a
    ALPP Antibody (N-term) (Cat. #AM2022a) western blot analysis in mouse placenta tissue lysates (35μg/lane).This demonstrates the ALPP antibody detected the ALPP protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05187
Other Accession P10696, NP_001623.3
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 421CT8.1.1
Calculated MW 57954 Da
Antigen Region 56-83 aa
Additional Information
Gene ID 250
Other Names Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALPP, PLAP
Target/Specificity This ALPP antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the N-terminal region of human ALPP.
Dilution IF~~1:100
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALPP Antibody (N-term)(Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms PLAP
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Location Detected in placenta (at protein level).
Research Areas
Citations (0)

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There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.


References for protein:
1.Stec, B., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 8), 866-870 (2010)
2.Wang, F., et al. Am. J. Surg. Pathol. 33(10):1529-1539(2009)
3.Estrada, K., et al. Hum. Mol. Genet. 18(18):3516-3524(2009)
4.Zhu, J.F., et al. Zhonghua Wai Ke Za Zhi 47(5):381-384(2009)
5.Roberson, J.R., et al. Pediatr Blood Cancer 51(6):840-842(2008)

References for HeLa cell line:

1. Scherer WF, Syverton JT, Gey GO (May 1953). "Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix". J. Exp. Med. 97 (5): 695–710. [PubMed:13052828].

2. Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). "Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping". Cancer Res. 59 (1): 141–50. [PubMed: 9892199].
3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). "A novel L1 retrotransposon marker for HeLa cell line identification". BioTechniques 46 (4): 277–84. [PubMed: 19450234].

4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). "Check your cultures! A list of cross-contaminated or misidentified cell lines". Int. J. Cancer 127 (1): 1–8. [PubMed:20143388].

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$ 295.00
Cat# AM2022a
(40 western blots)
Availability: In Stock
Bulk Size
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