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GABARAP Antibody(Ascites)

Mouse Monoclonal Antibody (Mab)

  • WB - GABARAP Antibody(Ascites) AM2025a
    All lanes : Anti- at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GABARAP Antibody(Ascites) AM2025a
    GABARAP Antibody (Cat. #AM2025a) western blot analysis in mouse brain tissue lysates (35μg/lane).This demonstrates the GABARAP antibody detected the GABARAP protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95166
Other Accession P60517, Q8MK68, Q9DCD6, Q9GJW7, NP_009209.1
Reactivity Human, Mouse
Predicted Bovine, Rabbit, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 424CT5.1.6
Calculated MW 13918 Da
Additional Information
Gene ID 11337
Other Names Gamma-aminobutyric acid receptor-associated protein, GABA(A) receptor-associated protein, MM46, GABARAP, FLC3B
Target/Specificity Purified His-tagged GABARAP protein(Fragment) was used to produced this monoclonal antibody.
Dilution WB~~1:500~2000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGABARAP Antibody(Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms FLC3B
Function Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Cellular Location Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome Note=Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity)
Tissue Location Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
Research Areas
Citations (0)

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Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.


Garcia-Martin, E., et al. J. Neurol. (2010) In press :
Komoike, Y., et al. J. Hum. Genet. 55(3):155-162(2010)
Thielmann, Y., et al. Proteins 77(3):637-646(2009)
Schwarten, M., et al. Autophagy 5(5):690-698(2009)
Kirkin, V., et al. Mol. Cell 33(4):505-516(2009)

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$ 295.00
Cat# AM2025a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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