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SPRED1 Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

  • WB - SPRED1 Antibody (Ascites) AM2036a
    SPRED1 Antibody (Cat. #AM2036a) western blot analysis in mouse kidney tissue lysates (35μg/lane).This demonstrates the SPRED1 antibody detected the SPRED1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q7Z699
Other Accession NP_689807.1
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 462CT2.5.1
Calculated MW 50477 Da
Additional Information
Gene ID 161742
Other Names Sprouty-related, EVH1 domain-containing protein 1, Spred-1, hSpred1, SPRED1
Target/Specificity Purified His-tagged SPRED1 protein(Fragment) was used to produced this monoclonal antibody.
Dilution WB~~1:100~1600
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPRED1 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow (By similarity).
Cellular Location Cell membrane; Peripheral membrane protein Membrane, caveola; Peripheral membrane protein. Nucleus. Note=Localized in cholesterol-rich membrane raft/caveola fractions
Tissue Location Weakly expressed in embryonic cell line HEK293.
Research Areas
Citations (0)

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The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq].


Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Batz, C., et al. Blood 115(12):2557-2558(2010)
Messiaen, L., et al. JAMA 302(19):2111-2118(2009)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Spurlock, G., et al. J. Med. Genet. 46(7):431-437(2009)

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$ 295.00
Cat# AM2036a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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