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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SOX2 Antibody (Ascites)   

SOX2 Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

  • IF - SOX2 Antibody (Ascites) AM2048b
    Fluorescent confocal image of an SY5Y cell stained with AM2048b SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AM2048b SOX2 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 3 min). SOX2 immunoreactivity is localized exclusively to the nucleus.
  • WB - SOX2 Antibody (Ascites) AM2048b
    Western blot analysis of anti-SOX2 Monoclonal Antibody(Cat.#AM2048b) by human SOX2 recombinant protein cell line lysate (35μg/lane). SOX2 (His tagged) (arrow) was detected using the purified Mab.
  • FC - SOX2 Antibody (Ascites) AM2048b
    Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (ascites)(bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P48431
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 57CT23.3.4
Calculated MW 34310 Da
Additional Information
Gene ID 6657
Other Names Transcription factor SOX-2, SOX2
Target/Specificity Human SOX2 recombinant protein is used to produce this monoclonal antibody.
Dilution IF~~1:200
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSOX2 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SOX2
Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)

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This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).


References for protein:
1.Effects of MCF2L2, ADIPOQ and SOX2 genetic polymorphisms on the development of nephropathy in type 1 Diabetes Mellitus. Zhang D, et al. BMC Med Genet, 2010 Jul 28. PMID 20667095.
2.Sox2 protein expression is an independent poor prognostic indicator in stage I lung adenocarcinoma. Sholl LM, et al. Am J Surg Pathol, 2010 Aug. PMID 20631605.
3.Sox2 is translationally activated by eukaryotic initiation factor 4E in human glioma-initiating cells. Ge Y, et al. Biochem Biophys Res Commun, 2010 Jul 9. PMID 20537983.
4.Mutational screening of CHX10, GDF6, OTX2, RAX and SOX2 genes in 50 unrelated microphthalmia-anophthalmia-coloboma (MAC) spectrum cases. Gonzalez-Rodriguez J, et al. Br J Ophthalmol, 2010 Aug. PMID 20494911.
5.Examination of SOX2 in variable ocular conditions identifies a recurrent deletion in microphthalmia and lack of mutations in other phenotypes. Reis LM, et al. Mol Vis, 2010 Apr 28. PMID 20454695.
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].

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Cat# AM2048b
(40 western blots)
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