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SOX2 Antibody (Ascites)Mouse Monoclonal Antibody (Mab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AM2048b | 0.1 ml 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
SOX2 Antibody (Ascites) - Product info | |
| Application | IF, WB, FC
|
| Primary Accession | P48431 |
| Reactivity | Human |
| Concentration | Ascites |
| Isotype | IgG1 |
| Clone Names | 57CT23.3.4 |
| Calculated MW | 34310 Da |
SOX2 Antibody (Ascites) - Additional info | |
| Gene ID 6657 | |
| Other Names SOX2; Transcription factor SOX-2 | |
| Target/Specificity Human SOX2 recombinant protein is used to produce this monoclonal antibody. | |
| Dilution IF~~1:200 WB~~1:200~2000 FC~~1:50~100 | |
| Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions SOX2 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures. | |
SOX2 Antibody (Ascites) - Protein Information | |
| Name SOX2 | |
| Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity) | |
| Cellular Location Nucleus. | |
SOX2 Antibody (Ascites) - Related products
AM2048b: SOX2 Antibody (Ascites)
AP2048b: SOX2 Antibody (C-term)
AP2048c: SOX2 Antibody (Center)
AP2048d: SOX2 Antibody (N-term)
AP3651a: Phospho-SOX2-S83 Antibody
AP3730a: Phospho-SOX2- Y108 Antibody
AP3736a: Phospho-Sox2- pS249 Antibody
AP3737a: phospho-Sox2- pS250 Antibody
AP3742a: phospho-Sox2- S251 Antibody
AP3750a: phospho-Sox2- S246 Antibody
AP5651a: Sox2-pS246-pS249-pS250--pS251 Antibody (C-term)
DC03709: Human SOX2 cDNA Clone
LY12065a: SOX2 Over-expression Lysate
BP2048b: SOX2 Antibody (C-term) Blocking Peptide
BP2048c: SOX2 Antibody (Center) Blocking Peptide
BP2048d: SOX2 Antibody (N-term) Blocking Peptide
BP2048e: SOX2 Antibody (C265) Blocking Peptide
BP2048g: SOX2 Antibody (A30) Blocking Peptide
BP3651a: Phospho-SOX2-S83 Antibody Blocking Peptide
BP3730a: Phospho-SOX2-Y108 Antibody Blocking peptide
BP5651a: Sox2-pS246-pS249-pS250--pS251 Antibody (C-term) Blocking peptide
SOX2 Antibody (Ascites) - Application data
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Fluorescent confocal image of an SY5Y cell stained with AM2048b SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AM2048b SOX2 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). SOX2 immunoreactivity is localized exclusively to the nucleus.
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Western blot analysis of anti-SOX2 Monoclonal Antibody(Cat.#AM2048b) by human SOX2 recombinant protein cell line lysate (35ug/lane). SOX2 (His tagged) (arrow) was detected using the purified Mab.
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Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (ascites)(bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
SOX2 Antibody (Ascites) - Research Areas
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BACKGROUND
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
REFERENCES
References for protein: 1.Effects of MCF2L2, ADIPOQ and SOX2 genetic polymorphisms on the development of nephropathy in type 1 Diabetes Mellitus. Zhang D, et al. BMC Med Genet, 2010 Jul 28. PMID 20667095. 2.Sox2 protein expression is an independent poor prognostic indicator in stage I lung adenocarcinoma. Sholl LM, et al. Am J Surg Pathol, 2010 Aug. PMID 20631605. 3.Sox2 is translationally activated by eukaryotic initiation factor 4E in human glioma-initiating cells. Ge Y, et al. Biochem Biophys Res Commun, 2010 Jul 9. PMID 20537983. 4.Mutational screening of CHX10, GDF6, OTX2, RAX and SOX2 genes in 50 unrelated microphthalmia-anophthalmia-coloboma (MAC) spectrum cases. Gonzalez-Rodriguez J, et al. Br J Ophthalmol, 2010 Aug. PMID 20494911. 5.Examination of SOX2 in variable ocular conditions identifies a recurrent deletion in microphthalmia and lack of mutations in other phenotypes. Reis LM, et al. Mol Vis, 2010 Apr 28. PMID 20454695. References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
