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FOXA1 Antibody (Center D159) (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • WB - FOXA1 Antibody (Center D159) (Ascites) AM2103a
    FOXA1 Antibody (Center D159)(Ascites)(Cat. #AM2103a) western blot analysis in Hela cell line lysates (35μg/lane).This demonstrates the FOXA1 antibody detected the FOXA1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P55317
Other Accession P23512, P35582, NP_004487
Reactivity Human
Predicted Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone/Animal Names 596CT18.4.3
Calculated MW 49148 Da
Antigen Region 143-171 aa
Additional Information
Gene ID 3169
Other Names Hepatocyte nuclear factor 3-alpha, HNF-3-alpha, HNF-3A, Forkhead box protein A1, Transcription factor 3A, TCF-3A, FOXA1, HNF3A, TCF3A
Target/Specificity This FOXA1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 143-171 amino acids from the Central region of human FOXA1.
Dilution WB~~1:500~1000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFOXA1 Antibody (Center D159) (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOXA1
Synonyms HNF3A, TCF3A
Function Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'- [AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00089, ECO:0000269|PubMed:15987773, ECO:0000269|PubMed:16331276}
Tissue Location Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas
Citations (0)
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Background

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

References

Hu, D.G., et al. Mol. Pharmacol. 78(4):714-722(2010)
Zhang, Y., et al. J. Biol. Chem. 285(37):28604-28613(2010)
Bernardo, G.M., et al. Development 137(12):2045-2054(2010)
Liu, N., et al. Zhonghua Yi Xue Za Zhi 90(20):1403-1407(2010)
Song, Y., et al. Cancer Res. 70(5):2115-2125(2010)

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$ 182.50
Cat# AM2103a
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