|Application ||WB, E|
|Calculated MW||13157 Da|
|Antigen Region||60-92 aa|
|Other Names||Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, MLANA, MART1|
|Target/Specificity||This MART-1/Melan-A antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 60-92 amino acids from the C-terminal region of human MART-1/Melan-A.|
|Format||Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MART-1/Melan-A Antibody (C-term) (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation|
|Tissue Location||Expression is restricted to melanoma and melanocyte cell lines and retina|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
MLANA is involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein SILV/PMEL17, which is critical to the formation of stage II melanosomes.
Li, Y., et al. J. Mol. Biol. 399(4):596-603(2010)
Giordano, F., et al. Hum. Mol. Genet. 18(23):4530-4545(2009)
Fernandez, L.P., et al. Exp. Dermatol. 18(7):634-642(2009)
Beltraminelli, H., et al. Am J Dermatopathol 31(3):305-308(2009)
Serana, F., et al. J Transl Med 7, 21 (2009) :
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