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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CD3 Antibody   

CD3 Antibody

Mouse Monoclonal Antibody (Mab)

     
  • FC - CD3 Antibody AM2153b
    Overlay histogram showing huuman peripheral blood lymphocytes stained with CD3 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37ºC. The secondary antibody used was Goat Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • SPECIFICATION
  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, E
Primary Accession P07766
Other Accession P09693, P20963
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names OKT3
Calculated MW 23147 Da
Additional Information
Gene ID 916
Other Names T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Target/Specificity This CD3 antibody is generated from mouse immunized with human CD3.
Dilution FC~~1:50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD3E
Synonyms T3E
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down- regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).
Cellular Location Cell membrane; Single-pass type I membrane protein
Research Areas
Citations ( 0 )

Background

 The CD3 complex mediates signal transduction.

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$ 295.00
$ 99.00
Cat# AM2153b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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