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SPARC Antibody(C-term) Antibody(Ascites)

Mouse Monoclonal Antibody (Mab)

  • WB - SPARC Antibody(C-term) Antibody(Ascites) AM2175a
    SPARC Antibody (C-term)(Ascites)(Cat. #AM2175a) western blot analysis in Hela cell line lysates (35μg/lane).This demonstrates the SPARC antibody detected the SPARC protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09486
Other Accession P36378, P16975, P36233, P20112, P07214, P36377, P13213, NP_003109
Reactivity Human
Predicted Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 664CT7.3.1
Calculated MW 34632 Da
Antigen Region 224-251 aa
Additional Information
Gene ID 6678
Other Names SPARC, Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine, SPARC, ON
Target/Specificity This SPARC antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC.
Dilution WB~~1:100~800
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPARC Antibody(C-term) Antibody(Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ON
Function Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Cellular Location Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane
Research Areas
Citations (0)

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Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM) (Bradshaw et al., 2003 [PubMed 12721366]).


Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Howard, C., et al. Histol. Histopathol. 25(9):1163-1169(2010)
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Liang, J.F., et al. J. Exp. Clin. Cancer Res. 29, 71 (2010) :

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$ 295.00
Cat# AM2175a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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