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ERCC1 Antibody (C-term)

Mouse Monoclonal Antibody (Mab)

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  • WB - ERCC1 Antibody (C-term) AM2186b
    All lanes : Anti-ERCC1 Antibody (C-term) at 1:2000 dilution Lane 1: A549 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: T47D whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ERCC1 Antibody (C-term) AM2186b
    Western blot analysis of lysates from A549, T47D cell line (from left to right), using ERCC1 Antibody (C-term)(Cat. #AM2186b). AM2186b was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
  • WB - ERCC1 Antibody (C-term) AM2186b
    ERCC1 Antibody (C-term) (Cat. #AM2186b) western blot analysis in Hela,A549 cell line lysates (35μg/lane).This demonstrates the ERCC1 antibody detected the ERCC1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P07992
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM,k
Clone Names 752CT13.2.5
Calculated MW 32562 Da
Antigen Region 268-297 aa
Additional Information
Gene ID 2067
Other Names DNA excision repair protein ERCC-1, ERCC1
Target/Specificity This ERCC1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 268-297 amino acids from the C-terminal region of human ERCC1.
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsERCC1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ERCC1
Function Isoform 1: Non-catalytic component of a structure- specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.
Cellular Location Isoform 1: Nucleus. Isoform 3: Nucleus.
Research Areas
Citations (0)

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Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.


van Duin M., et al. Cell 44:913-923(1986).
Hoeijmakers J.H.J., et al. Cold Spring Harb. Symp. Quant. Biol. 51:91-101(1986).
Yu J.J., et al. Mutat. Res. 382:13-20(1997).
Hisatomi H., et al. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AM2186b
(40 western blots)
Availability: In Stock
Bulk Size
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