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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ENO1 Antibody   

ENO1 Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • IHC-P - ENO1 Antibody AM2192b
    Immunohistochemical analysis of paraffin-embedded H. kideny section using ENO1 Antibody(Cat#AM2192b). AM2192b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • WB - ENO1 Antibody AM2192b
    ENO1 Antibody (Cat. #AM2192b) western blot analysis in MCF-7,A431,Hela,HepG2,mouse NIH/3T3 cell line and mouse stomach,liver tissue lysates (35μg/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P06733
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG (k)
Clone Names 786CT6.6.4
Calculated MW 47169 Da
Additional Information
Gene ID 2023
Other Names Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, Plasminogen-binding protein, ENO1, ENO1L1, MBPB1, MPB1
Target/Specificity Purified His-tagged ENO1 protein was used to produced this monoclonal antibody.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsENO1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ENO1
Synonyms ENO1L1, MBPB1, MPB1
Function Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
Cellular Location Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line
Tissue Location The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons
Research Areas
Citations (0)

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Background

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

References

Giallongo A., et al. Proc. Natl. Acad. Sci. U.S.A. 83:6741-6745(1986).
Giallongo A., et al. Eur. J. Biochem. 190:567-573(1990).
Ray R., et al. Mol. Cell. Biol. 11:2154-2161(1991).
Walter M., et al. J. Autoimmun. 8:931-945(1995).
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AM2192b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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