|Application ||WB, E|
|Calculated MW||32844 Da|
|Other Names||Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3, MBD3|
|Target/Specificity||Purified His-tagged MBD3 protein was used to produced this monoclonal antibody.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MBD3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.|
|Cellular Location||Nucleus. Chromosome. Note=Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes|
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Provided below are standard protocols that you may find useful for product applications.
Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
Hendrich B., et al. Mol. Cell. Biol. 18:6538-6547(1998).
Ota T., et al. Nat. Genet. 36:40-45(2004).
Grimwood J., et al. Nature 428:529-535(2004).
Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Tatematsu K., et al. Genes Cells 5:677-688(2000).
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