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UCHL1 Antibody (C-term)

Mouse Monoclonal Antibody (Mab)

     
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  • IHC-P - UCHL1 Antibody (C-term) AM2207b
    Immunohistochemical analysis of paraffin-embedded H. brain section using UCHL1 Antibody (C-term)(Cat#AM2207B). AM2207B was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • IHC-P - UCHL1 Antibody (C-term) AM2207b
    Immunohistochemical analysis of paraffin-embedded H. astroglioma section using UCHL1 Antibody (C-term)(Cat#AM2207B). AM2207B was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • WB - UCHL1 Antibody (C-term) AM2207b
    UCHL1 Antibody (C-term)(Cat. #AM2207b) western blot analysis in 293T,NCI-H1299,rat PC-12 cell line lysates (35μg/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P09936
Other Accession Q00981, Q6SEG5, Q9R0P9, Q60HC8, P23356, Q9GM50
Reactivity Human, Rat
Predicted Bovine, Horse, Monkey, Mouse, Pig
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 696CT2.1.4
Calculated MW 24824 Da
Additional Information
Gene ID 7345
Other Names Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, 6---, Neuron cytoplasmic protein 95, PGP 95, PGP95, Ubiquitin thioesterase L1, UCHL1
Target/Specificity This UCHL1 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the 185-214 region of human UCHL1.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUCHL1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UCHL1
Function Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Cellular Location Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain
Tissue Location Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients
Research Areas
Citations (0)

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Background

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

References

Boudreaux D.A., et al. Proc. Natl. Acad. Sci. U.S.A. 107:9117-9122(2010).
Hillier L.W., et al. Nature 434:724-731(2005).
Mural R.J., et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Day I.N.M., et al. Biochem. J. 268:521-524(1990).
Choi J., et al. J. Biol. Chem. 279:13256-13264(2004).

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$ 295.00
$ 99.00
Cat# AM2207b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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