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CCRK Antibody (N-term)

Mouse Monoclonal Antibody (Mab)

     
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  • WB - CCRK Antibody (N-term) AM2214b
    CCRK Antibody (N-term)(Cat. #AM2214b) western blot analysis in MCF-7,A549 cell line lysates (35μg/lane).This demonstrates the CCRK antibody detected the CCRK protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8IZL9
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone/Animal Names 885CT27.1.1
Calculated MW 38695 Da
Additional Information
Gene ID 23552
Other Names Cyclin-dependent kinase 20, CDK-activating kinase p42, CAK-kinase p42, Cell cycle-related kinase, Cell division protein kinase 20, Cyclin-dependent protein kinase H, Cyclin-kinase-activating kinase p42, CDK20, CCRK, CDCH
Target/Specificity Purified His-tagged CCRK protein was used to produced this monoclonal antibody.
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCRK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDK20
Synonyms CCRK, CDCH
Function Required for high-level Shh responses in the developing neural tube. Together with TBC1D32, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling (By similarity). Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160'.
Cellular Location Nucleus. Cytoplasm. Cell projection, cilium
Research Areas
Citations (0)
citation

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Background

Required for high-level Shh responses in the developing neural tube. Together with BROMI, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling (By similarity). Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160'.

References

Liu Y., et al. J. Biol. Chem. 279:4507-4514(2004).
Wang L.Y., et al. Oncogene 31:2907-2918(2012).
Qiu H., et al. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
Ota T., et al. Nat. Genet. 36:40-45(2004).
Humphray S.J., et al. Nature 429:369-374(2004).

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$ 365.00
$ 140.00
Cat# AM2214b
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