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>   home   >   Products   >   Primary Antibodies   >   Cardiovascular   >   CD73(NT5E) Antibody (C-term)   

CD73(NT5E) Antibody (C-term)

Mouse Monoclonal Antibody (Mab)

     
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  • IHC-P - CD73(NT5E) Antibody (C-term) AM2223a
    AM2223A staining CD73 (NT5E) in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - CD73(NT5E) Antibody (C-term) AM2223a
    AM2223A staining CD73 (NT5E) in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - CD73(NT5E) Antibody (C-term) AM2223a
    Anti-CD73 (NT5E) Antibody (C-term) at 1:4000 dilution + MDA-MB-231 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CD73(NT5E) Antibody (C-term) AM2223a
    Anti-CD73 (NT5E) Antibody (C-term) at 1:2000 dilution + WiDr whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CD73(NT5E) Antibody (C-term) AM2223a
    Anti-CD73 (NT5E) Antibody (C-term) at 1:4000 dilution + MDA-MB-231 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P21589
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 974CT7.5.3
Calculated MW 63368 Da
Additional Information
Gene ID 4907
Other Names 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE
Target/Specificity Purified His-tagged CD73(NT5E) protein was used to produced this monoclonal antibody.
Dilution IHC-P~~1:25
WB~~1:5000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD73(NT5E) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NT5E
Synonyms NT5, NTE
Function Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Research Areas
Citations (0)

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Background

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

References

Misumi Y., et al. Eur. J. Biochem. 191:563-569(1990).
Otsuki T., et al. DNA Res. 12:117-126(2005).
Mungall A.J., et al. Nature 425:805-811(2003).
Hansen K.R., et al. Gene 167:307-312(1995).
Zanoni L., et al. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
Cat# AM2223a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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