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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   VRK1 Antibody (Center)(Ascites)   

VRK1 Antibody (Center)(Ascites)

Mouse Monoclonal Antibody (Mab)

     
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  • WB - VRK1 Antibody (Center)(Ascites) AM2226a
    VRK1 Antibody (Center)(Cat. #AM2226a) western blot analysis in Hela,HL-60 cell line lysates (35μg/lane).This demonstrates the VRK1 antibody detected the VRK1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q99986
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 1015CT2.1.1
Calculated MW 45476 Da
Additional Information
Gene ID 7443
Other Names Serine/threonine-protein kinase VRK1, Vaccinia-related kinase 1, VRK1
Target/Specificity Purified His-tagged VRK1 protein was used to produced this monoclonal antibody.
Dilution WB~~1:5000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVRK1 Antibody (Center)(Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VRK1
Function Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr- 18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis
Tissue Location Widely expressed. Highly expressed in fetal liver, testis and thymus.
Research Areas
Citations (0)

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Background

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm.

References

Nezu J., et al. Genomics 45:327-331(1997).
Lopez-Borges S., et al. Oncogene 19:3656-3664(2000).
Barcia R., et al. Arch. Biochem. Biophys. 399:1-5(2002).
Nichols R.J., et al. J. Biol. Chem. 279:7934-7946(2004).
Blanco S., et al. FEBS J. 273:2487-2504(2006).

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$ 295.00
Cat# AM2226a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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