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VRK1 Antibody (Center)(Ascites)
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q99986 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone/Animal Names | 1015CT2.1.1 |
| Calculated MW | 45476 Da |
| Gene ID | 7443 |
|---|---|
| Other Names | Serine/threonine-protein kinase VRK1, Vaccinia-related kinase 1, VRK1 |
| Target/Specificity | Purified His-tagged VRK1 protein was used to produced this monoclonal antibody. |
| Dilution | WB~~1:5000 |
| Format | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | VRK1 Antibody (Center)(Ascites) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | VRK1 {ECO:0000303|PubMed:9344656, ECO:0000312|HGNC:HGNC:12718} |
|---|---|
| Function | Serine/threonine kinase involved in cell cycle, nuclear condensation and transcription regulation (PubMed:14645249, PubMed:18617507, PubMed:19103756). Involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation (PubMed:19103756). Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2 (PubMed:10951572). Phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity (PubMed:33076429). Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm (PubMed:16495336). Phosphorylates ATF2 which activates its transcriptional activity (PubMed:15105425). |
| Cellular Location | Nucleus. Cytoplasm. Note=Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis |
| Tissue Location | Widely expressed. Highly expressed in fetal liver, testis and thymus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm.
References
Nezu J., et al. Genomics 45:327-331(1997).
Lopez-Borges S., et al. Oncogene 19:3656-3664(2000).
Barcia R., et al. Arch. Biochem. Biophys. 399:1-5(2002).
Nichols R.J., et al. J. Biol. Chem. 279:7934-7946(2004).
Blanco S., et al. FEBS J. 273:2487-2504(2006).
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