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POU2AF1 Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • WB - POU2AF1 Antibody AM2243b
    POU2AF1 Antibody (Cat. #AM2243b) western blot analysis in Daudi cell line lysates (35μg/lane).This demonstrates the POU2AF1 antibody detected the POU2AF1 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q16633
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,κ
Clone Names 1170CT1.1.1
Calculated MW 27436 Da
Additional Information
Gene ID 5450
Other Names POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1, POU2AF1, OBF1
Target/Specificity Purified His-tagged POU2AF1 protein was used to produced this monoclonal antibody.
Dilution WB~~1:4000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOU2AF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POU2AF1
Synonyms OBF1
Function Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Cellular Location Nucleus.
Tissue Location B-cell specific.
Research Areas
Citations (0)

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Background

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

References

Strubin M., et al. Cell 80:497-506(1995).
Gstaiger M., et al. Nature 373:360-362(1995).
Galiegue-Zouitina S., et al. C. R. Acad. Sci. III, Sci. Vie 318:1125-1131(1995).
Luo Y., et al. Mol. Cell. Biol. 15:4115-4124(1995).
Ota T., et al. Nat. Genet. 36:40-45(2004).

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$ 295.00
$ 99.00
Cat# AM2243b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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