- CITATIONS: 2
|Application ||WB, IF, FC, E|
|Calculated MW||54671 Da|
|Other Names||Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4, EZF, GKLF|
|Target/Specificity||KLF4 recombinant protein is used to produce this monoclonal antibody.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KLF4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.|
Provided below are standard protocols that you may find useful for product applications.
MCC-555-induced NAG-1 expression is mediated in part by KLF4. Cekanova M, et al. Eur J Pharmacol, 2010 Jul 10. PMID 20385121. Kruppel-like factor 4 inhibits epithelial-to-mesenchymal transition through regulation of E-cadherin gene expression. Yori JL, et al. J Biol Chem, 2010 May 28. PMID 20356845. MicroRNA-10b promotes migration and invasion through KLF4 in human esophageal cancer cell lines. Tian Y, et al. J Biol Chem, 2010 Mar 12. PMID 20075075. Genetic and epigenetic inactivation of Kruppel-like factor 4 in medulloblastoma. Nakahara Y, et al. Neoplasia, 2010 Jan. PMID 20072650. Kruppel-like factor 4 (Klf4) prevents embryonic stem (ES) cell differentiation by regulating Nanog gene expression. Zhang P, et al. J Biol Chem, 2010 Mar 19. PMID 20071344.
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