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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EGFR Antibody (Ascites)   

EGFR Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

  • WB - EGFR Antibody (Ascites) AM7628a
    Western blot analysis of anti-EGFR Monoclonal Antibody (Cat.#AM7628a) by EGFR recombinant protein (Fragment). EGFR (Fragment) protein (arrow) was detected using the ascites Mab. (1:2000)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00533
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1κ
Clone Names 51CT78.40.5
Calculated MW 134277 Da
Additional Information
Gene ID 1956
Other Names Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Target/Specificity Purified His-tagged EGFR protein(Fragment) was used to produced this monoclonal antibody.
Dilution WB~~1:2000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEGFR Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ERBB, ERBB1, HER1
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Cellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF)
Tissue Location Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Research Areas
Citations ( 0 )


The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.


Complex Mutations in the Epidermal Growth Factor Receptor Gene in Non-small Cell Lung Cancer. Hata A, et al. J Thorac Oncol, 2010 Aug 30. PMID 20808254. EGFR signaling is differentially activated in patient-derived glioblastoma stem cells. Howard BM, et al. J Exp Ther Oncol, 2010. PMID 20734923. [EGFR Mutations Detection in Non-small Cell Lung Cancer Tissues by Real-time PCR and DNA Sequencing.] Li Y, et al. Zhongguo Fei Ai Za Zhi, 2009 Dec 20. PMID 20723379. [Detection and Its Clinical Significance of EGFR Gene Mutation and Gene Amplification in 187 Patients with Non-small Cell Lung Cancer.] Liu H, et al. Zhongguo Fei Ai Za Zhi, 2009 Dec 20. PMID 20723374. Effect of gefitinib on the survival of patients with recurrence of lung adenocarcinoma after surgery: A retrospective case-matching cohort study. Katayama T, et al. Surg Oncol, 2010 Aug 10. PMID 20705455.

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$ 295.00
Cat# AM7628a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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