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MAFK Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • FC - MAFK Antibody AM8420b
    Flow cytometric analysis of Hela cells using MAFK Antibody(green, Cat#AM8420b) compared to an isotype control of mouse IgG1(blue). AM8420b was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
  • WB - MAFK Antibody AM8420b
    Western blot analysis of lysate from Jurkat cell line, using MAFK Antibody(Cat. #AM8420b). AM8420b was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35μg.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession O60675
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,κ
Clone Names 1328CT786.105.125
Calculated MW 17523 Da
Additional Information
Gene ID 7975
Other Names Transcription factor MafK, Erythroid transcription factor NF-E2 p18 subunit, MAFK
Target/Specificity This MAFK antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human MAFK.
Dilution FC~~1:25
WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAFK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAFK
Function Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.
Cellular Location Nucleus.
Research Areas
Citations (0)

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Background

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.

References

Toki T.,et al.Oncogene 14:1901-1910(1997).
Hillier L.W.,et al.Nature 424:157-164(2003).
Daub H.,et al.Mol. Cell 31:438-448(2008).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Mayya V.,et al.Sci. Signal. 2:RA46-RA46(2009).

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$ 295.00
$ 99.00
Cat# AM8420b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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