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>   home   >   Products   >   Primary Antibodies   >   Crown Antibodies   >   IF4E Antibody   

IF4E Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • IF - IF4E Antibody AM8471b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with AM8471b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
  • WB - IF4E Antibody AM8471b
    All lanes : Anti-IF4E Antibody at 1:4000 dilution Lane 1: Hela whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: K562 whole cell lysates Lane 4: NIH/3T3 whole cell lysates Lane 5: PC-12 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession P06730
Reactivity Human, Mouse, Rat
Host Mouse
Clonality monoclonal
Isotype IgG1,Κ
Clone Names 1504CT774.14.55
Calculated MW 25097 Da
Additional Information
Gene ID 1977
Other Names Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F
Target/Specificity This IF4E antibody is generated from a mouse immunized with a recombinant protein of human IF4E.
Dilution IF~~1:25
WB~~1:4000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIF4E Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF4E
Synonyms EIF4EL1, EIF4F
Function Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Cellular Location Cytoplasm, P-body. Cytoplasm
Research Areas
Citations (0)

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Background

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

References

Rychlik W.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:945-949(1987).
Rychlik W.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:1148-1148(1992).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Oh J.H.,et al.Mamm. Genome 16:942-954(2005).

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$ 295.00
$ 99.00
Cat# AM8471b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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