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MGMT Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • FC - MGMT Antibody AM8481b
    Overlay histogram showing Jurkat cells stained with AM8481b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8481b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - MGMT Antibody AM8481b
    All lanes : Anti-MGMT Antibody at1:1000-1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: MOLT-4 whole cell lysate Lane 4: Raji whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P16455
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1527CT158.87.44
Calculated MW 21646 Da
Additional Information
Gene ID 4255
Other Names Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT
Target/Specificity This MGMT antibody is generated from a mouse immunized with a recombinant protein.
Dilution FC~~1:25
WB~~1:1000-1:2000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMGMT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MGMT
Function Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Cellular Location Nucleus.
Citations (0)

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Background

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

References

Tano K.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:686-690(1990).
Rydberg B.,et al.J. Biol. Chem. 265:9563-9569(1990).
Koike G.,et al.J. Biol. Chem. 265:14754-14762(1990).
Hayakawa H.,et al.J. Mol. Biol. 213:739-747(1990).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AM8481b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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