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MPIP3 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - MPIP3 Antibody AM8486b
    All lanes : Anti-MPIP3 Antibody at 1:1000-1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: Raji whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - MPIP3 Antibody AM8486b
    AM8486b staining MPIP3 in human testis sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P30307
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG2a,κ
Clone/Animal Names 1535CT627.6.80
Calculated MW 53365 Da
Additional Information
Gene ID 995
Other Names M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C
Target/Specificity This MPIP3 antibody is generated from a mouse immunized with a recombinant protein.
Dilution WB~~1:1000-1:2000
IHC-P~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMPIP3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC25C
Function Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Cellular Location Nucleus
Research Areas
Citations (0)
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Background

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.

References

Sadhu K.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:5139-5143(1990).
Bureik M.,et al.Int. J. Oncol. 17:1251-1258(2000).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Wegener S.,et al.Eur. J. Cell Biol. 79:810-815(2000).
Hernandez S.,et al.Lab. Invest. 81:465-473(2001).

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$ 365.00
$ 140.00
Cat# AM8486b
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