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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SODM Antibody   

SODM Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • FC - SODM Antibody AM8491b
    Overlay histogram showing A549 cells stained with AM8491b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8491b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • IHC-P - SODM Antibody AM8491b
    AM8491b staining SODM in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - SODM Antibody AM8491b
    AM8491b staining SODM in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - SODM Antibody AM8491b
    All lanes : Anti-SODM Antibody at 1:2000 dilution Lane 1: human brain lysate Lane 2: Hela whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P04179
Reactivity Human, Mouse
Host Mouse
Clonality monoclonal
Isotype IgG1,κ
Clone Names 1559CT572.3.83
Calculated MW 24722 Da
Additional Information
Gene ID 6648
Other Names Superoxide dismutase [Mn], mitochondrial, SOD2
Target/Specificity This SODM antibody is generated from a mouse immunized with a recombinant protein of human SODM.
Dilution FC~~1:25
IHC-P~~1:25
WB~~1:2000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSODM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SOD2
Function Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Cellular Location Mitochondrion matrix.
Research Areas
Citations (0)

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Background

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

References

Wispe J.R.,et al.Biochim. Biophys. Acta 994:30-36(1989).
Beck Y.,et al.Nucleic Acids Res. 15:9076-9076(1987).
Heckl K.,et al.Nucleic Acids Res. 16:6224-6224(1988).
Ho Y.-S.,et al.FEBS Lett. 229:256-260(1988).
Church S.L.,et al.Biochim. Biophys. Acta 1087:250-252(1990).

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$ 295.00
$ 99.00
Cat# AM8491b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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