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RAB18 Antibody

Purified Mouse Monoclonal Antibody (Mab)

  • FC - RAB18 Antibody AM8510b
    Overlay histogram showing PC-3 cells stained with AM8510b(green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8510b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - RAB18 Antibody AM8510b
    All lanes : Anti-RAB18 Antibody at 1:500-1:2000 dilution Lane 1: PC-3 whole cell lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lane 4: human brain lysate Lane 5: human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NP72
Other Accession Q5R5H5
Reactivity Human, Mouse, Rat
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1573CT811.119.39
Calculated MW 22977 Da
Additional Information
Gene ID 22931
Other Names Ras-related protein Rab-18, RAB18
Target/Specificity This RAB18 antibody is generated from a mouse immunized with a recombinant protein of human RAB18.
Dilution FC~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB18 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB18
Function Plays a role in apical endocytosis/recycling. May be implicated in transport between the plasma membrane and early endosomes. Plays a key role in eye and brain development and neurodegeneration.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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Plays a role in apical endocytosis/recycling. May be implicated in transport between the plasma membrane and early endosomes. Plays a key role in eye and brain development and neurodegeneration.


Chikri M.M.,et al.Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
Schaefer U.,et al.FEBS Lett. 466:148-154(2000).
Dou T.,et al.DNA Seq. 16:230-234(2005).
Cui W.C.,et al.Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AM8510b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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