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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RAB27A Antibody   

RAB27A Antibody

Purified Mouse Monoclonal Antibody (Mab)

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  • FC - RAB27A Antibody AM8511b
    Overlay histogram showing K562 cells stained with AM8511b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8511b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - RAB27A Antibody AM8511b
    Anti-RAB27A Antibody at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P51159
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1590CT813.266.26
Calculated MW 24868 Da
Additional Information
Gene ID 5873
Other Names Ras-related protein Rab-27A, Rab-27, GTP-binding protein Ram, RAB27A, RAB27
Target/Specificity This RAB27A antibody is generated from a mouse immunized with a recombinant protein of human RAB27A.
Dilution FC~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB27A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB27A
Synonyms RAB27
Function Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Cellular Location Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles
Tissue Location Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
Research Areas
Citations (0)

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Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.


Seabra M.C.,et al.J. Biol. Chem. 270:24420-24427(1995).
Seabra M.C.,et al.Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
Chen D.,et al.Biochem. Mol. Med. 60:27-37(1997).
Tolmachova T.,et al.Gene 239:109-116(1999).
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).

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$ 295.00
$ 99.00
Cat# AM8511b
(40 western blots)
Availability: In Stock
Bulk Size
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