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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RAB5C Antibody   

RAB5C Antibody

Purified Mouse Monoclonal Antibody (Mab)

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  • FC - RAB5C Antibody AM8520b
    Overlay histogram showing U-2OS cells stained with AM8520b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8520b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - RAB5C Antibody AM8520b
    All lanes : Anti-RAB5C Antibody at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P51148
Other Accession Q5R7L7
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG2b,k
Clone Names 1616CT314.65.31
Calculated MW 23483 Da
Additional Information
Gene ID 5878
Other Names Ras-related protein Rab-5C, L1880, RAB5L, RAB5C, RABL
Target/Specificity This RAB5C antibody is generated from a mouse immunized with a recombinant protein of human RAB5C.
Dilution FC~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB5C Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB5C
Synonyms RABL
Function Protein transport. Probably involved in vesicular traffic (By similarity).
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Research Areas
Citations (0)

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Protein transport. Probably involved in vesicular traffic (By similarity).


Han H.J.,et al.Cytogenet. Cell Genet. 73:137-139(1996).
Albertsen H.M.,et al.Nat. Genet. 7:472-479(1994).
Clemens D.L.,et al.Infect. Immun. 68:2671-2684(2000).
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 295.00
$ 99.00
Cat# AM8520b
(40 western blots)
Availability: In Stock
Bulk Size
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