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BANP Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - BANP Antibody AM8526b
    Anti-BANP Antibody at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - BANP Antibody AM8526b
    AM8526b staining BANP in human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q8N9N5
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,κ
Clone/Animal Names 1524CT337.6.32
Calculated MW 56494 Da
Additional Information
Gene ID 54971
Other Names Protein BANP, BEN domain-containing protein 1, Btg3-associated nuclear protein, Scaffold/matrix-associated region-1-binding protein, BANP, BEND1, SMAR1
Target/Specificity This BANP antibody is generated from a mouse immunized with a recombinant protein between 30-390 amino acids from human BANP.
Dilution WB~~1:1000
IHC-P~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBANP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BANP
Synonyms BEND1, SMAR1
Function Controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function. Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 activation, which causes cell cycle arrest (By similarity).
Cellular Location Nucleus.
Tissue Location Down-regulated in breast cancer cell lines.
Citations (0)
citation

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Background

Controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function. Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 'Ser-15' phosphorylation and nuclear accumulation, which causes cell cycle arrest (By similarity).

References

Ota T.,et al.Nat. Genet. 36:40-45(2004).
Martin J.,et al.Nature 432:988-994(2004).
Birot A.-M.,et al.Gene 253:189-196(2000).
Rampalli S.,et al.Mol. Cell. Biol. 25:8415-8429(2005).
Olsen J.V.,et al.Sci. Signal. 3:RA3-RA3(2010).

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$ 182.50
$ 70.00
Cat# AM8526b
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