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GARS Antibody

Purified Mouse Monoclonal Antibody (Mab)

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  • FC - GARS Antibody AM8548b
    Overlay histogram showing Hela cells stained with AM8548b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8548b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - GARS Antibody AM8548b
    All lanes : Anti-GARS Antibody at 1:4000 dilution Lane 1: Ramos whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: HT-1080 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P41250
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1641CT125.1.1
Calculated MW 83166 Da
Additional Information
Gene ID 2617
Other Names Glycine--tRNA ligase,, Diadenosine tetraphosphate synthetase, AP-4-A synthetase, Glycyl-tRNA synthetase, GlyRS, GARS
Target/Specificity This GARS antibody is generated from a mouse immunized with a recombinant protein between 35-305 amino acids from human GARS.
Dilution FC~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGARS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.
Cellular Location Cytoplasm. Mitochondrion. Cell projection, axon Note=Associated with granules in cultured neuron cells
Tissue Location Widely expressed, including brain and spinal cord.
Citations (0)

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Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.


Shiba K.,et al.J. Biol. Chem. 269:30049-30055(1994).
Williams J.H.,et al.Nucleic Acids Res. 23:1307-1310(1995).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 424:157-164(2003).
Ge Q.,et al.J. Biol. Chem. 269:28790-28797(1994).

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$ 295.00
$ 99.00
Cat# AM8548b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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