|Application ||WB, E|
|Calculated MW||85653 Da|
|Other Names||Targeting protein for Xklp2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL-2, Hepatocellular carcinoma-associated antigen 519, Hepatocellular carcinoma-associated antigen 90, Protein fls353, Restricted expression proliferation-associated protein 100, p100, TPX2, C20orf1, C20orf2, DIL2, HCA519|
|Target/Specificity||This TPX2 antibody is generated from a mouse immunized with a recombinant protein between 1-531 amino acids from the human TPX2.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TPX2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||C20orf1, C20orf2, DIL2, HCA519|
|Function||Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin- alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940).|
|Cellular Location||Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole Note=During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.|
|Tissue Location||Expressed in lung carcinoma cell lines but not in normal lung tissues|
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Provided below are standard protocols that you may find useful for product applications.
Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
Manda R.,et al.Genomics 61:5-14(1999).
Zhang Y.,et al.Cytogenet. Cell Genet. 84:182-183(1999).
Nezu J.,et al.Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
Wang Y.,et al.J. Immunol. 169:1102-1109(2002).
Deloukas P.,et al.Nature 414:865-871(2001).
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