|Application ||WB, E|
|Calculated MW||85653 Da|
|Other Names||Targeting protein for Xklp2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL-2, Hepatocellular carcinoma-associated antigen 519, Hepatocellular carcinoma-associated antigen 90, Protein fls353, Restricted expression proliferation-associated protein 100, p100, TPX2, C20orf1, C20orf2, DIL2, HCA519|
|Target/Specificity||This TPX2 antibody is generated from a mouse immunized with a recombinant protein between 1-531 amino acids from the human TPX2.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TPX2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||C20orf1, C20orf2, DIL2, HCA519|
|Function||Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin- alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940).|
|Cellular Location||Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole Note=During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.|
|Tissue Location||Expressed in lung carcinoma cell lines but not in normal lung tissues|
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Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
Manda R.,et al.Genomics 61:5-14(1999).
Zhang Y.,et al.Cytogenet. Cell Genet. 84:182-183(1999).
Nezu J.,et al.Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
Wang Y.,et al.J. Immunol. 169:1102-1109(2002).
Deloukas P.,et al.Nature 414:865-871(2001).
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