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>   home   >   Products   >   Primary Antibodies   >   Crown Antibodies   >   PRDM16 Antibody   

PRDM16 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - PRDM16 Antibody AM8585b
    All lanes : Anti-PRDM16 Antibody at 1:4000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 140 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9HAZ2
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1740CT793.52.44
Calculated MW 140251 Da
Additional Information
Gene ID 63976
Other Names PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1, MDS1/EVI1-like gene 1, PRDM16, KIAA1675, MEL1, PFM13
Target/Specificity This PRDM16 antibody is generated from a mouse immunized with a recombinant protein between 779-996 amino acids from PRDM16.
Dilution WB~~1:4000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRDM16 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRDM16 (HGNC:14000)
Function Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation.
Cellular Location Nucleus.
Tissue Location Expressed in uterus and kidney. Expressed in both cardiomyocytes and interstitial cells
Research Areas
Citations (0)

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Background

Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation.

References

Mochizuki N.,et al.Blood 96:3209-3214(2000).
Fang W.,et al.Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
Nagase T.,et al.DNA Res. 7:347-355(2000).
Nakajima D.,et al.DNA Res. 9:99-106(2002).
Gregory S.G.,et al.Nature 441:315-321(2006).

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$ 295.00
$ 99.00
Cat# AM8585b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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