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EI2BG Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • FC - EI2BG Antibody AM8608b
    Overlay histogram showing U-2 OS cells stained with AM8608b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8608b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NH174309) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - EI2BG Antibody AM8608b
    All lanes : Anti-EI2BG Antibody at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q9NR50
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1778CT889.66.6.54
Calculated MW 50240 Da
Additional Information
Gene ID 8891
Other Names Translation initiation factor eIF-2B subunit gamma, eIF-2B GDP-GTP exchange factor subunit gamma, EIF2B3
Target/Specificity This EI2BG antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 110-452 amino acids from human EI2BG.
Dilution FC~~1:25
WB~~1:2000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEI2BG Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF2B3
Function Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Research Areas
Citations (0)

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Background

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

References

Krueger M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:8566-8571(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AM8608b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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Ordering Information

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