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human CD25 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • FC - human CD25 Antibody AM8656b
    Overlay histogram showing PHA-stimulated huuman peripheral blood lymphocytes stained with CD25 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37ºC. The secondary antibody used was Goat Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, E
Primary Accession unknow
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG2a
Clone Names 7G7B6 Equivalent
Additional Information
Dilution FC~~1:50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionshuman CD25 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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$ 295.00
$ 99.00
Cat# AM8656b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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Ordering Information

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