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Phospho-Thr286 CaM Kinase II Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Thr286 CaM Kinase II Antibody AN1001
    Western blot of rat brain lysate showing specific immunolabeling of the ~50k α- and the ~60k β-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with λ-Ptase.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11275
Reactivity Rat
Predicted Human, Mouse, Xenopus
Host Rabbit
Clonality polyclonal
Calculated MW 50/60 KDa
Additional Information
Gene ID 25400
Gene Name CAMK2A/B
Other Names Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, Camk2a
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~50k α-CaM Kinase II and the ~60k β-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the λ-phosphatase treatment.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Thr286 CaM Kinase II Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002).


Elgersma Y, Fedorov NB, Ikonen S, Choi ES, Elgersma M, Carvalho OM, Giese KP, Silva AJ (2002) Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning. Neuron 36:493-505.
Gleason MR, Higashijima S, Dallman J, Liu K, Mandel G, Fetcho JR (2003) Translocation of CaM kinase II to synaptic sites in vivo. Nature Neurosci 6:217-218.
Hudmon A, Schulman H (2002) Neuronal Ca2+/calmodulin-dependent protein kinase II: The role of structure and autoregulation in cellular function. Annu Rev Biochem 71:473-510.
Kennedy MB (1998) Signal transduction molecules at the glutamatergic postsynaptic membrane. Brain Res Rev 26:243-257.
Meng FJ, Guo J, Zhang QG, Song B, Zhang GY (2003) Autophosphorylated calcium/calmodulin-dependent protein kinase IIa (CaMKIIa) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in cerebral ischemia and reperfusion in hippocampus of rats. Brain Res 967:161-169.
Picciotto MR, Czernik AJ, Nairn AC (1993) Calcium/calmodulin-dependent protein kinase I. cDNA cloning and identification of autophosphorylation site. J Biol Chem 268:26512-26521.
Schulman H, Hanson PI (1993) Multifunctional Ca2+/calmodulin-dependent protein kinase. Neurochem Res 18:65-77.
Soderling TR (2000) CaM-kinases: modulators of synaptic plasticity. Curr Opin Neurobiol 10:375-380.
Sun X, Milovanovic M, Zhao Y, Wolf ME. (2008) Acute and chronic dopamine receptor stimulation modulates AMPA receptor trafficking in nucleus accumbens neurons cocultured with prefrontal cortex neurons. J Neurosci. Apr 16;28(16):4216-30.
Rebekah S. Vest, Heather O'Leary, Steven J. Coultrap, Mark S. Kindy, and K. Ulrich Bayer (2010) Effective Post-insult Neuroprotection by a Novel Ca2+/ Calmodulin-dependent Protein Kinase II (CaMKII) InhibitorJ. Biol. Chem., 285: 20675 - 20682.
Kurtis D. Davies, Susan M. Goebel-Goody, Steven J. Coultrap, and Michael D. Browning (2008) Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization J. Biol. Chem., 283: 33138 - 33146.
Xiu Sun, Michael Milovanovic, Yun Zhao, and Marina E. Wolf (2008) Acute and Chronic DopamineStimulation Modulates AMPA Receptor Trafficking in Nucleus Accumbens Neurons Cocultured with Prefrontal Cortex Neurons. J. Neurosci., 28: 4216 – 4230
Rebekah S. Vest, Kurtis D. Davies, Heather O'Leary, J. David Port, and K. Ulrich Bayer (2007) Dual Mechanism of a Natural CaMKII Inhibitor. Mol. Biol. Cell, 18: 5024 - 5033.
Matthew Townsend, Tapan Mehta, and Dennis J. Selkoe (2007) Soluble A Inhibits Specific Signal Transduction Cascades Common to the Insulin Receptor Pathway. J. Biol. Chem., 282: 33305 - 33312

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$ 335.00
Cat# AN1001
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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