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Phospho-Tyr15 cdc2 Antibody
Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P39951 |
| Reactivity | Human, Xenopus |
| Predicted | Mouse, Rat, Zebrafish |
| Host | Rabbit |
| Clonality | polyclonal |
| Calculated MW | 34 KDa |
| Gene ID | 54237 |
|---|---|
| Gene Name | CDK1 |
| Other Names | Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, Cdk1, Cdc2, Cdc2a, Cdkn1 |
| Target/Specificity | Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 conjugated to KLH. |
| Dilution | WB~~ 1:1000 |
| Format | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. |
| Antibody Specificity | Specific for the ~38k cdc2 protein phosphorylated at Tyr15. Immunolabeling is blocked by the λ-phosphatase treatment. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-Tyr15 cdc2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle (Maller, 1991). The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2 (Nakamizo et al., 2002). cdc2 expression in brain has been linked to both neurogenesis and apoptosis (Konishi and Bonni, 2003; Dranovsky et al., 2001; Okano et al., 1996).
References
Dranovsky A, Vincent I, Gregori L, Schwarzman A, Colflesh D, Enghild J, Strittmatter W, Davies P, Goldgaber D (2001) cdc2 phosphorylation of nucleolin demarcates mitotic stages and Alzheimer's disease pathology. Neurobiol Aging 22:517-528.
Konishi Y, Bonni A (2003) The E2F-cdc2 cell-cycle pathway specifically mediates activity deprivation-induced apoptosis of postmitotic neurons. J Neurosci 23:1649-1658.
Maller JL (1991) Mitotic control. Curr Opin Cell Biol 3:269-275.
Nakamizo A, Inamura T, Inoha S, Amano T, Ochi H, Ikezaki K, Fukui M (2002) Suppression of cdc2 dephosphorylation at the tyrosine 15 residue during nitrosourea-induced G2M phase arrest in glioblastoma cell lines. J Neurooncol 59:7-13.
Okano HJ, Pfaff DW, Gibbs RB (1996) Expression of EGFR-, p75NGFR-, and PSTAIR (cdc2)-like immunoreactivity by proliferating cells in the adult rat hippocampal formation and forebrain. Dev Neurosci 18:199-209.
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