Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   Phospho-Ser774 Dynamin Antibody   

Phospho-Ser774 Dynamin Antibody

Affinity purified sheep polyclonal antibody

  • WB - Phospho-Ser774 Dynamin Antibody AN1007
    Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with λ-Ptase.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21575
Reactivity Rat
Predicted Bovine, Chicken, Human, Mouse, Monkey
Host Sheep
Clonality polyclonal
Calculated MW 95 KDa
Additional Information
Gene ID 140694
Gene Name DNM1
Other Names Dynamin-1, B-dynamin, D100, Dynamin, brain, Dnm1, Dnm
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from sheep serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~95k dynamin protein phosphorylated at Ser774. Labelsthe purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react withother purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blockedby λ-phosphatase treatment.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser774 Dynamin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of Ser774 by GSK3 has recently been shown to control activity-dependent bulk endocytosis of synaptic vesicles (Clayton et al., 2010)


Cousin MA, Tan TC, Robinson PJ (2001) Protein phosphorylation is required for endocytosis in nerve terminals:
potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin. J Neurochem
Graham ME, O'Callaghan DW, McMahon HT, Burgoyne RD (2002) Dynamin-dependent and dynamin-independent
processes contribute to the regulation of single vesicle release kinetics and quantal size. Proc Natl Acad Sci USA
Tan TC, Valova VA, Malladi CS, Graham ME, Berven LA, Jupp OJ, Hansra G, McClure SJ, Sarcevic B, Boadle RA,
Larsen MR, Cousin MA, Robinson PJ (2003) cdk5 is essential for synaptic vesicle endocytosis. Nat Cell Biol
Tsuboi T, Terakawa S, Scalettar BA, Fantus C, Roder J, Jeromin A (2002) Sweeping model of dynamin activity -
Visualization of coupling between exocytosis and endocytosis under an evanescent wave microscope with green
fluorescent proteins. J Biol Chem 277:15957-15961.
Clayton EL, Sue N, Smillie KJ, O'Leary T, Bache N, Cheung G, Cole AR, Wyllie DJ, Sutherland C, Robinson PJ,
Cousin MA. (2010) Dynamin I phosphorylation by GSK3 controls activity-dependent bulk endocytosis of synaptic
vesicles. Nat Neurosci. 13(7):845-51.
Sergio Leal-Ortiz, Clarissa L. Waites, Ryan Terry-Lorenzo, Pedro Zamorano, Eckart D. Gundelfinger, and Craig C.
Garner (2008) Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. J. Cell Biol., 181:
831 - 846.

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

$ 335.00
Cat# AN1007
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions