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Phospho-Thr202/Tyr204 ERK/MAPK Antibody
Affinity purified rabbit polyclonal antibody
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Application 
| WB, IHC |
|---|---|
| Primary Accession | P63086 |
| Reactivity | Human, Rat |
| Predicted | Bovine, Chicken, Mouse, Monkey, Xenopus, Zebrafish |
| Host | Rabbit |
| Clonality | polyclonal |
| Calculated MW | 42/44 KDa |
| Gene ID | 116590 |
|---|---|
| Gene Name | MAPK1/2 |
| Other Names | Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, Mapk1, Erk2, Mapk, Prkm1 |
| Target/Specificity | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH. |
| Dilution | WB~~ 1:1000 IHC~~ 1:500 |
| Format | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. |
| Antibody Specificity | Specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by λ-phosphatase. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-Thr202/Tyr204 ERK/MAPK Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
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Provided below are standard protocols that you may find useful for product applications.
Background
Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).
References
Adams JP, Sweatt JD (2002) Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 42:135-163.
Ahn, NG (1993) The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem 127-128:201-209.
Johnson GL, Lapadat R (2002) Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298:1911-1912.
Tanoue TJ, Nishida, E (2003) Molecular recognitions in the MAP kinase cascades. Cellular Signaling 15:455-462.
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