|Calculated MW||90/120 KDa|
|Other Names||Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3, PGR, NR3C3|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser294 conjugated to KLH.|
|Format||Prepared by affinity purification using a protein G column.|
|Antibody Specificity||Specific for the ~90k PR-A isoform and the ~120k PR-B isoformphosphorylated at Ser294. Immunolabeling is blocked by the phosphopeptide used as theantigen but not by the corresponding dephosphopeptide.|
|Precautions||Phospho-Ser294 Progesterone Receptor Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000; Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000) . Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser 190 and Ser 294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.
Attia GR, Zeitoun K, Edwards D, Johns A, Carr BR, Bulun SE (2000) Progesterone receptor isoform A but not B is expressed in
endometriosis. J Clin Endocrinol Metab 85:2897-2902.
Clemm DL, Sherman L, Boonyara
tanakornkit V, Schrader WT, Weigel NL, Edwa
rds DP (2000) Differential hormone-dependent
phosphorylation of progesterone receptor A and B forms reveal
ed by a phosphoserine site-specific monoclonal antibody.
Mol Endocrinol 14:52-65.
Edwards DP (2005) Regulation of signal transduction pathwa
ys by estrogen and progesterone. Annu Rev Physiol 67:335-376.
Lin VC, Woon CT, Aw SE, Guo C (2003) Distinct molecular path
ways mediate progesterone-induced growth inhibition and
focal adhesion. Endocrinology 144:5650-5657.
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