|Application ||WB, IHC|
|Predicted||Bovine, Chicken, Human, Mouse, Monkey, Zebrafish|
|Calculated MW||60/62 KDa|
|Other Names||Synaptotagmin-1, Synaptotagmin I, SytI, p65, Syt1|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202 conjugated to KLH.|
|Dilution||WB~~ 1:1000 |
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns|
|Antibody Specificity||Specific for the ~60k - 62k synaptotagmin protein phosphorylated at Thr202. The immunolabeling is completely eliminated by λ-phosphatase treatment. In some lysates and/or various tissues, additional bands may be seen at ~ 45k, 75k and 150k.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Thr202 Synaptotagmin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).
Fernandez-Chacon R, Konigstorfer A, Gerber SH, Garcia J, Matos MF, Stevens CF, Brose N, Rizo J, Rosenmund C, Sudhof TC (2001) Synaptotagmin I functions as a calcium regulator of release probability. Nature (London) 410:41-49.
Hilfiker S, Pieribone VA, Nordstedt C, Greengard P, Czernik AJ (1999) Regulation of synaptotagmin I phosphorylation by multiple protein kinases. J Neurochem 73:921-932.
Lee BH, Min X, Heise CJ, Xu BE, Chen S, Shu H, Luby-Phelps K, Goldsmith EJ, Cobb MH (2004) WNK1 phosphorylates synaptotagmin 2 and modulates its membrane binding. Mol Cell 15:741-751.
Poskanzer KE, Marek KW, Sweeney ST, Davis GW (2003) Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo. Nature (London) 426:559-563.
Wang CT, Lu JC, Bai JH, Chang PY, Martin TFJ, Chapman ER, Jackson MB (2003) Different domains of synaptotagmin control the choice between kiss-and-run and full fusion. Nature (London) 424:943-947.
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