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Dopamine β-Hydroxylase, C-Terminus Antibody

Affinity purified sheep polyclonal antibody

     
  • WB - Dopamine β-Hydroxylase, C-Terminus Antibody AN1033
    Western blot of human adrenal medulla lysate showing specific immunolabeling of the ~75k DBH protein.
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  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P09172
Reactivity Human, Mouse
Host Sheep
Clonality polyclonal
Calculated MW 75 KDa
Additional Information
Gene ID 1621
Gene Name DBH
Other Names Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, Soluble dopamine beta-hydroxylase, DBH
Target/Specificity Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from sheep serum by affinity purification using a Sulfo-Link® column matrix to which the peptide immunogen was coupled.
Antibody Specificity Specific for the ~75k DBH protein in Western blots.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDopamine β-Hydroxylase, C-Terminus Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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citation

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Background

DBH catalyzes the conversion of dopamine to norepinephrine and serves as a marker of noradrenergic cells. DBH antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). The expression of DBH is also elevated during stress (Sabban and Kvetnansky, 2001).

References

Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
Sabban EL, Kvetnansky R (2001) Stress-triggered activation of gene expression in catecholaminergic systems: dynamics of transcriptional events. Trends Neurosci 24:91-98.
Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.
Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286.

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$ 365.00
Cat# AN1033
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