NMDA Receptor, NR1 Subunit, C2 Splice Variant Insert Antibody
Affinity purified rabbit polyclonal antibody
|Calculated MW||120 KDa|
|Other Names||Glutamate receptor ionotropic, NMDA 1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1, Grin1, Nmdar1|
|Target/Specificity||Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, C2 splice variant insert conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.|
|Antibody Specificity||Specific for the ~120k NR1 subunit of the NMDA Receptor containing the C2 splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2 insert.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NMDA Receptor, NR1 Subunit, C2 Splice Variant Insert Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1 subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1 subunit generates up to eight NR1 subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.
Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
Foldes RL, Rampersad V, Kamboj RK (1994) Cloning and sequence analysis of additional splice variants encoding human N-methyl-D-aspartate receptor (hNR1) subunits. Gene 147:303-304.
Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358.
Zukin RS, Bennett MVL (1995) Alternatively spliced isoforms of the NMDARI receptor subunit. Trends Neurosci 18:306-313.
Mannie M. Y. Fan, Herman B. Fernandes, Lily Y. J. Zhang, Michael R. Hayden, and Lynn A. Raymond (2007) Altered NMDA Receptor Trafficking in a Yeast Artificial Chromosome Transgenic Mouse Model of Huntington's DiseaseJ. Neurosci., 27: 3768 - 3779.
Note: Dr. Michael Browning, a co-author of one of the cited papers, is President and founder of PhosphoSolutions.
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