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Synapsin I Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Synapsin I  Antibody AN1059
    Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~78k synapsin I doublet protein.
  • IF - Synapsin I  Antibody AN1059
    Immunostaining of cultured rat caudate neurons showing punctate distribution of synapsin in green and MAP in red. Cells and photo courtesy of QBMCellScience.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17599
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Calculated MW 78 KDa
Additional Information
Gene ID 281510
Gene Name SYN1
Other Names Synapsin-1, Synapsin I, SYN1
Target/Specificity Native protein purified from bovine brain.
Dilution WB~~ 1:1000
IF~~ 1:1000
Format Prepared from rabbit serum by affinity purification using a column to which the native protein was coupled.
Antibody Specificity Specific for the ~78k synapsin I doublet in Western blots of rat brainextracts. Immunolabeling blocked by preadsorption of antibody with the protein used togenerate the antibody.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSynapsin I Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
Citations (0)

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Colorado Biosciences Park 12635 East Montview Boulevard, #213 Aurora, CO 80045-7337 Tel: (888) 442-7100 Page 1 of 2 WB = Western Blot IF = Immunofluorescence IHC = Immunohistochemistry IP = Immunoprecipitation Packaging: 10 μ g antibody vial; lyophilized from 5 mM ammonium bicarbonate . The antibody should be reconstituted in 50 μl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na 2 HPO 4 , 2.7 mM KCl, 1.5 mM KH 2 PO 4 , pH 7.4) before use. After reconstitution the antibody should be aliquoted and stored at -20 o C. Adequate amount of material to conduct 10-mini Western Blots. Storage and Stability: For long term storage –20 o C is recommended. Stable at –20 o C for at least 1 year Shipment: Domestic - Ambient; International – Ambient. Anti-Synapsin I Catalog Number: 1925-SYNP Size : 10 μg Product Description: Affinity purified rabbit polyclonal antibody Applications: WB : 1:1000 IHC/IF: 1:1000 IP : 1 μg per 200 μg lysate Antigen: Native protein purified from bovine brain. Species reactivity : The antibody has been directly tested for reactivity in Western blots with rat, mouse and human tissue. Biological Significance:


Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Fe
rreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA,
Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372-
Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Gr
eengard P, Czernik AJ (2001) Opposing changes in
phosphorylation of specific sites in synapsin I during Ca
-dependent glutamate release in isolated nerve
terminals. J Neurosci 21:7944-7953.
Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M,
Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A
protein kinase A-dependent molecular switch in synapsin
s regulates neurite outgrowth. Nature Neurosci 5:431-
Moore RY, Bernstein M (1989) Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron
microscopy and synapsin I immunoreactivity. J Neurosci 9:2151-2162.
Nayak AS, Moore CI, Browning MD (1996) CaM kinase II
phosphorylation of the presyn
aptic protein synapsin is
persistently increased during expression of long-term po
tentiation. Proc Natl Acad Sci (USA) 93:15451-15456.
Stone LM, Browning MD, Finger TE (1994) Differential dist
ribution of the synapsins in the rat olfactory bulb. J
Neurosci 14:301-309.
Kurtis D. Davies, Susan M. Goebel-Goody, St
even J. Coultrap, and Michael D. Browning (2008)
Long Term Synaptic
Depression That Is Associated with GluR1 Dephosphorylation but Not
isoxazolepropionic Acid (AMPA) Receptor Internalization
J. Biol. Chem.,
283: 33138 - 33146.
Note: Dr. Michael Browning, co-author of the
cited papers is the President and founder

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$ 335.00
Cat# AN1059
(40 western blots)
Availability: 7-10 days
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