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Synapsin I Antibody
Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF |
|---|---|
| Primary Accession | P17599 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | polyclonal |
| Calculated MW | 78 KDa |
| Gene ID | 281510 |
|---|---|
| Gene Name | SYN1 |
| Other Names | Synapsin-1, Synapsin I, SYN1 |
| Target/Specificity | Native protein purified from bovine brain. |
| Dilution | WB~~ 1:1000 IF~~ 1:1000 |
| Format | Prepared from rabbit serum by affinity purification using a column to which the native protein was coupled. |
| Antibody Specificity | Specific for the ~78k synapsin I doublet in Western blots of rat brainextracts. Immunolabeling blocked by preadsorption of antibody with the protein used togenerate the antibody. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Synapsin I Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Colorado Biosciences Park 12635 East Montview Boulevard, #213 Aurora, CO 80045-7337 Tel: (888) 442-7100 Page 1 of 2 WB = Western Blot IF = Immunofluorescence IHC = Immunohistochemistry IP = Immunoprecipitation Packaging: 10 μ g antibody vial; lyophilized from 5 mM ammonium bicarbonate . The antibody should be reconstituted in 50 μl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na 2 HPO 4 , 2.7 mM KCl, 1.5 mM KH 2 PO 4 , pH 7.4) before use. After reconstitution the antibody should be aliquoted and stored at -20 o C. Adequate amount of material to conduct 10-mini Western Blots. Storage and Stability: For long term storage –20 o C is recommended. Stable at –20 o C for at least 1 year Shipment: Domestic - Ambient; International – Ambient. Anti-Synapsin I Catalog Number: 1925-SYNP Size : 10 μg Product Description: Affinity purified rabbit polyclonal antibody Applications: WB : 1:1000 IHC/IF: 1:1000 IP : 1 μg per 200 μg lysate Antigen: Native protein purified from bovine brain. Species reactivity : The antibody has been directly tested for reactivity in Western blots with rat, mouse and human tissue. Biological Significance:
References
Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Fe
rreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA,
Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372-
4380.
Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Gr
eengard P, Czernik AJ (2001) Opposing changes in
phosphorylation of specific sites in synapsin I during Ca
2+
-dependent glutamate release in isolated nerve
terminals. J Neurosci 21:7944-7953.
Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M,
Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A
protein kinase A-dependent molecular switch in synapsin
s regulates neurite outgrowth. Nature Neurosci 5:431-
437.
Moore RY, Bernstein M (1989) Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron
microscopy and synapsin I immunoreactivity. J Neurosci 9:2151-2162.
Nayak AS, Moore CI, Browning MD (1996) CaM kinase II
phosphorylation of the presyn
aptic protein synapsin is
persistently increased during expression of long-term po
tentiation. Proc Natl Acad Sci (USA) 93:15451-15456.
Stone LM, Browning MD, Finger TE (1994) Differential dist
ribution of the synapsins in the rat olfactory bulb. J
Neurosci 14:301-309.
Kurtis D. Davies, Susan M. Goebel-Goody, St
even J. Coultrap, and Michael D. Browning (2008)
Long Term Synaptic
Depression That Is Associated with GluR1 Dephosphorylation but Not
-Amino-3-hydroxy-5-methyl-4-
isoxazolepropionic Acid (AMPA) Receptor Internalization
J. Biol. Chem.,
283: 33138 - 33146.
Note: Dr. Michael Browning, co-author of the
cited papers is the President and founder
PhosphoSolutions.
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