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Dopamine Transporter, Extracellular Loop 2 Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Dopamine Transporter, Extracellular Loop 2 Antibody AN1069
    Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01959
Reactivity Human, Monkey
Host Rabbit
Clonality polyclonal
Calculated MW 88 KDa
Additional Information
Gene ID 6531
Gene Name SLC6A3
Other Names Sodium-dependent dopamine transporter, DA transporter, DAT, Solute carrier family 6 member 3, SLC6A3, DAT1
Target/Specificity Synthetic peptide corresponding to amino acid residues from the intracellular C-terminal region conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification using a SulfoLink® column matrix to which the peptide immunogen was coupled.
Antibody Specificity Specific for the ~88k DAT protein in Western blots.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDopamine Transporter, Extracellular Loop 2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
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The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).


Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
Wilson JM, Kalasinsky KS, Levey AI, Bergeron C, Reiber G, Anthony RM, Schmunk GA, Shannak K, Haycock JW, Kish SJ (1996) Striatal dopamine nerve terminal markers in human, chronic methamphetamine users. Nat Med 2:699-703.
Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.
Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286.

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$ 335.00
Cat# AN1069
(40 western blots)
Availability: 7-10 days
Bulk Size
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