|Predicted||Mouse, Rat, Xenopus|
|Calculated MW||83 KDa|
|Other Names||Catenin beta-1, Beta-catenin, CTNNB1, CTNNB|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser33/37 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~83k β-catenin phosphorylated at Ser33 and Ser37|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Ser33,37 β-Catenin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
β-catenin is a central component of the cadherin cell adhesion complex and plays an essential role in neural development in the Wingless/Wnt signaling pathway (Ding and Dale, 2002; Polakis, 2000). The role of β-catenin in these processes is thought regulated by the sequential phosphorylation of Ser29 Ser33, Ser37 and Thr41 by glycogen synthase kinase 3β (GSK3β) (Liu et al., 2002). This hyperphosphorylation promotes the ubiquitylation and targeted destruction of β-catenin. Mutations in components of this phosphorylation regulated process that prevent β-catenin hyperphosphorylation by GSK3β are trongly associated with cancers (Wang et al., 2003; Polakis, 2000; Liu et al., 2002).
Ding Y, Dale, T (2002) Wnt signal transduction: kinase cogs in a nano-machine? Trends Biochem Sci 27:327-329.
Liu C, Li Y, Semenov M, Han C, Baeg GH, Tan Y, Zhang Z, Lin X, He X (2002) Control of β-catenin phosphorylation/degradation by a dual-kinase mechanism. Cell 108:837-847.
Polakis P (2000) Wnt signaling and cancer. Genes Dev 14:1837-1851.
Wang ZH, Vogelstein B, Kinzler KW (2003) Phosphorylation of β-catenin at S33, S37, or T41 can occur in the absence of phosphorylation at T45 in colon cancer cells. Cancer Res 63:5234-5235.
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