Phospho-Ser642 Raf-1 Antibody
Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession | P11345 |
Reactivity | Rat |
Predicted | Human, Mouse, Monkey, Chicken, Bovine |
Host | Rabbit |
Clonality | polyclonal |
Calculated MW | 74 KDa |
Gene ID | 24703 |
---|---|
Gene Name | RAF1 |
Other Names | RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, Raf1, Raf |
Target/Specificity | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser642 conjugated to KLH. |
Format | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns. |
Antibody Specificity | Specific for the ~74k Raf-1 protein phosphorylated at Ser642 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Phospho-Ser642 Raf-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Shipping | Blue Ice |
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Provided below are standard protocols that you may find useful for product applications.
Background
The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have s hown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser 301 and Ser 642
References
Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulati
ng the Raf kinase family. Cellular Signalling 15:463-469.
Dhillon AS, Kolch W (2002) Untying the regulation of
the Raf-1 kinase. Arch Biochem Biophys 404:3-9.
Dougherty MK, Muller J, Ritt DA, Zhou M, Zhou XZ, Copeland TD, Conrads TP, Veenstra TD, Lu KP, Morrison DK
(2005) Regulation of Raf-1 by Direct Feed
back Phosphorylation. Mol Cell 17:215-224.
Marshall CJ (1996) Ras effectors. current
opinion in cell biology 8:197-204.
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