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Vesicular GABA Amino Acid Transporter (VGAT) Antibody

Affinity purified rabbit polyclonal antibody

     
  • WB - Vesicular GABA Amino Acid Transporter (VGAT) Antibody AN1111
    Western blot of rat olfactory bulb lysate showing specific immunolabeling of the ~53k VGAT protein.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O35458
Reactivity Rat
Predicted Bovine, Monkey
Host Rabbit
Clonality polyclonal
Calculated MW 53 KDa
Additional Information
Gene ID 83612
Gene Name SLC32A1
Other Names Vesicular inhibitory amino acid transporter, GABA and glycine transporter, Solute carrier family 32 member 1, Vesicular GABA transporter, rGVAT, Slc32a1, Vgat, Viaat
Target/Specificity Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification on a column made with the peptide used as antigen.
Antibody Specificity Specific for the ~53k VGAT protein.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVesicular GABA Amino Acid Transporter (VGAT) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Background

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

References

Chaudhry FA, Reimer RJ, Bellocchio EE, Danbolt NC, Osen KK, Edwards RH, Storm-Mathisen J (1998) The vesicular GABA transporter, VGAT, localizes to synaptic vesicles in sets of glycinergic as well as GABAergic neurons. J Neurosci 18:9733-9750.
De GS, Schafer MK, Defamie N, Chen C, Ricci A, Weihe E, Varoqui H, Erickson JD (2005) Homeostatic scaling of vesicular glutamate and GABA transporter expression in rat neocortical circuits. J Neurosci 25:7121-7133.
McIntire SL, Reimer RJ, Schuske K, Edwards RH, Jorgensen EM (1997) Identification and characterization of the vesicular GABA transporter. Nature (Lond ) 389:870-876.

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$ 335.00
Cat# AN1111
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