|Calculated MW||82 KDa|
|Other Names||Rabphilin-3A, Exophilin-1, Rph3a|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~82k rabphilin protein phosphorylated at Ser234. Theimmunolabeling of the rabphilin band was completely blocked by the phosphopeptide used asantigen while the dephosphopeptide had no effect on the immunolabeling|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Ser234 Rabphilin 3A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994;Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggest ed to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995;Foletti and Scheller, 2001).
Foletti DL, Scheller RH (2001) Developmental regulation and specific brain distribution of
phosphorabphilin. J Neurosci 21:5461-5472.
Fykse EM, Li C, Sudhof TC (1995) Phosphorylatio
n of rabphilin-3A by Ca2+/calmodulin- and cAMP-
dependent protein kinases in vitro. J Neurosci 15:2385-2395.
Geppert M, Bolshakov VY, Siegelbaum SA, Takei K,
De Camilli P, Hammer RE, Südhof TC (1994) The
role of Rab3A in neurotransmitter release. Nature (Lond ) 369:493-497.
Li C, Takei K, Geppert M, Daniell L, Stenius K,
Chapman ER, Jahn R, De Camilli P, Südhof TC (1994)
Synaptic targeting of rabphilin
-3A, a synaptic vesicle Ca
/phospholipid- binding protein, depends on
rab3A/3C. Neuron 13:885-898.
Smith R, Petersen A, Bates GP, Brundin P, Li JY (20
05) Depletion of rabphilin 3A in a transgenic mouse
model (R6/1) of Huntington's disease, a possible
culprit in synaptic dysfunction. Neurobiol Dis 20:673-
Sergio Leal-Ortiz, Clarissa L. Waites, Ryan Terry-L
orenzo, Pedro Zamorano, Eckart D. Gundelfinger, and
Craig C. Garner (2008)
Piccolo modulation of Synapsin1a dy
namics regulates synaptic vesicle
J. Cell Biol., 181: 831 - 846
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