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Phospho-Ser9 GSK3β Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Ser9 GSK3β Antibody AN1117
    Western blot of rat cortex lysate showing specific immunolabeling of the ~46k GSK3βprotein phosphorylated at Ser9 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P18266
Reactivity Rat
Predicted Bovine, Chicken, Human, Mouse, Monkey, Xenopus, Zebrafish
Host Rabbit
Clonality polyclonal
Calculated MW 46 KDa
Additional Information
Gene ID 84027
Gene Name GSK3B
Other Names Glycogen synthase kinase-3 beta, GSK-3 beta, Factor A, FA, Serine/threonine-protein kinase GSK3B, Gsk3b
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~46k GSK3β protein phosphorylated at Ser9. Also weakly labels the ~51k GSK3α band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser9 GSK3β Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3α and GSK3β. Specifically, GSK3β has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser9 phosphorylation underlies the inhibition of GSK3β by insulin (Sutherland et al., 1993).


Sutherland C, Leighton IA, Cohen P (1993) Inactivation of glycogen synthase kinase 3 beta by phosphorylation: new kinase connections in insulin and growth factor signaling. Biochem J. Nov 15;
296 (Pt. 1):15-9.
Papkoff J, Aikawa M (1998) WNT-1 and HGF regulate GSK3b activity and beta catenin signaling in mammary epithelial cells. Biochem Biophys Res Commun. Jun 29;247(3):851-8.

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$ 335.00
Cat# AN1117
(40 western blots)
Availability: 7-10 days
Bulk Size
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