|Predicted||Bovine, Chicken, Human, Mouse, Monkey, Xenopus, Zebrafish|
|Calculated MW||46 KDa|
|Other Names||Glycogen synthase kinase-3 beta, GSK-3 beta, Factor A, FA, Serine/threonine-protein kinase GSK3B, Gsk3b|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~46k GSK3β protein phosphorylated at Ser9. Also weakly labels the ~51k GSK3α band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Ser9 GSK3β Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3α and GSK3β. Specifically, GSK3β has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser9 phosphorylation underlies the inhibition of GSK3β by insulin (Sutherland et al., 1993).
Sutherland C, Leighton IA, Cohen P (1993) Inactivation of glycogen synthase kinase 3 beta by phosphorylation: new kinase connections in insulin and growth factor signaling. Biochem J. Nov 15;
296 (Pt. 1):15-9.
Papkoff J, Aikawa M (1998) WNT-1 and HGF regulate GSK3b activity and beta catenin signaling in mammary epithelial cells. Biochem Biophys Res Commun. Jun 29;247(3):851-8.
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