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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Amyotrophic Lateral Sclerosis (ALS)   >   Phospho-Ser1480 NMDA Receptor NR2B Subunit Antibody   

Phospho-Ser1480 NMDA Receptor NR2B Subunit Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Ser1480 NMDA Receptor NR2B Subunit Antibody AN1127
    Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Ser1480 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser1480 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with λ-Ptase.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q00960
Reactivity Rat
Predicted Bovine, Chicken, Human, Mouse, Monkey, Xenopus
Host Rabbit
Clonality polyclonal
Calculated MW 180 KDa
Additional Information
Gene ID 24410
Gene Name GRIN2B
Other Names Glutamate receptor ionotropic, NMDA 2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, Grin2b
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1480 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for ~180k NMDAR NR2B subunit protein phosphorylated atSer1480. Immunolabeling of the NMDA NR2B subunit band is blocked by the phosphopeptideused as the antigen but not by the corresponding dephosphopeptide. Immunolabeling is alsoblocked by λ-phosphatase treatment.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser1480 NMDA Receptor NR2B Subunit Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
Citations (0)

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The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). It has been shown that phosphorylation of Ser1480 disrupts the interaction of NR2B with the PDZ domains of PSD-95 and SAP102 and decreases surface NR2B expression in neurons (Chung et al., 2004).


Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and
plasticity. Trends Neurosci 25:571-577.
Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but
not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
Ishii T, Moriyoshi K, Sugihara H, Sakurada K, Kadotani H, Yokoi M, Akazawa C, Shigemoto R, Mizuno N, Masu M,
Nakanishi S (1993) Molecular characterization of the family of the N-methyl- D-aspartate receptor subunits. J Biol
Chem 268:2836-2843.
Takasu, MA, Dalva, MB, Zigmond, RE, Greenberg, ME (2002) Modulation of NMDA Receptor -Dependent Calcium
Influx and Gene Expression Through EphB Receptors. Science 295:491-495.
Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev
Pharmacol Toxicol 43:335-358.
Chung HJ, Huang YH, Lau LF, Huganir RL (2004) Regulation of the NMDA receptor complex and trafficking by
activity-dependent phosphorylation of the NR2B subunit PDZ ligand. J. Neurosci. 24(45): 10248-10259.

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$ 335.00
Cat# AN1127
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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