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Fibrillarin (Nop1p) Antibody

Mouse monoclonal antibody

  • WB - Fibrillarin (Nop1p) Antibody AN1141
    Western blot of HeLa lysate showing specific immunolabeling of the ~ 34k fibrillarin protein.
  • IF - Fibrillarin (Nop1p) Antibody AN1141
    Human SH-SY5Y cells stained with mouse-anti-fibrillarin, showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15646
Reactivity Human, Rat
Host mouse
Clonality monoclonal
Isotype IgG1
Clone Names 38F3
Calculated MW 34 KDa
Additional Information
Gene ID 851548
Gene Name NOP1
Other Names rRNA 2'-O-methyltransferase fibrillarin, 211-, Histone-glutamine methyltransferase, U3 small nucleolar RNA-associated protein NOP1, Nucleolar protein 1, U3 snoRNA-associated protein NOP1, NOP1, LOT3
Target/Specificity Yeast nuclear preparations.
Dilution WB~~ 1:1000
IF~~ 1:500
Format Total IgG fraction
Antibody Specificity Specific for the ~34kDa Fibrillarin /Nop1p protein.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFibrillarin (Nop1p) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).


1. Ochs RL, Lischwe MA, Spohn WH, Busch H. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biol Cell 54:123-133 (1985).
2. Aris JP and Blobel G. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein. J. Cell Biol. 107:17-31 (1988).
3. Newton K, Petfalski E, Tollervey D, Caceres JF. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol Cell Biol. 23:8519-8527 (2003).
4. Tyagi S and Alsmadi O. Imaging native beta-actin mRNA in motile fibroblasts. Biophys J. 87:4153-62 (2004).
5. Paeschke1 K, Simonsson T, Postberg J, Rhodes D, Lipps H-J. Telomere end-binding proteins control the formation of G-quadruplex DNA structures in vivo Nature Structural & Molecular Biology 12, 847-854 (2005).
6. Vermaak D, Henikoff S, Malik HS. Positive selection drives the evolution of rhino, a member of the heterochromatin protein 1 family in Drosophila. PLoS Genetics 1:96-108 (2005).

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$ 285.00
Cat# AN1141
(40 western blots)
Availability: 7-10 days
Bulk Size
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