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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   Neurofilament L (NF-L) Antibody   

Neurofilament L (NF-L) Antibody

Mouse monoclonal antibody

  • WB - Neurofilament L (NF-L) Antibody AN1147
    Western blot of rat cortex lysate showing specificimmunolableing of the ~ 68k NF-L protein.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02547
Reactivity Rat
Predicted Chicken, Human, Mouse
Host Mouse
Clonality monoclonal
Isotype IgG1
Clone Names DA2
Calculated MW 68 KDa
Additional Information
Gene ID 4747
Gene Name NEFL
Other Names Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein, NEFL
Target/Specificity Preparation of porcine NF-L.
Dilution WB~~ 1:1000
Format Unpurified, concentrated culture supernatant.
Antibody Specificity Specific for the ~68k Neurofilament L protein.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNeurofilament L (NF-L) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
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Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2).


1. Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal
tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
2. Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin
AN and Evgrafov OV. (2000) A new variant of Charcot-Marie-Tooth disease type 2 is
probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet.

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$ 285.00
Cat# AN1147
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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